A Patient with Philadelphia-Negative Acute Lymphoblastic Leukemia with a FISH-Negative Cryptic BCR-ABL1 Rearrangement Detected by PCR and Sequencing Analysis

A Patient with Philadelphia-Negative Acute Lymphoblastic Leukemia with a FISH-Negative Cryptic BCR-ABL1 Rearrangement Detected by PCR and Sequencing Analysis

Nuri Lee1,4, Hyewon Lee2, Ji Yeon Sohn1, Ja-Hyun Jang5, Hyeon-Seok Eom2 and SunYoung Kong1,3* 1Department of Laboratory Medicine, National Cancer Center, Korea 2Hematologic Oncology Clinic, National Cancer Center, Korea 3Translational Epidemiology Research Branch, National Cancer Center, Korea 4Department of Laboratory Medicine, Seoul National University Hospital, Korea 5Green Cross Laboratorie...

FISH-negative, cytogenetically cryptic acute promyelocytic leukemia

Approximately 600–800 new cases of acute promyelocytic leukemia (APL) occur annually in the United States. The advent of all-trans retinoic acid (ATRA) has converted this subtype of acute leukemia to a readily curable one with excellent long-term outcomes. Rapid diagnosis and immediate treatment is crucial in APL and a requirement for favorable prognosis. t(15;17)(q24;q21) is the characteristic...

Identification of Mixed Lineage Leukemia Gene (MLL)/MLLT10 Fusion Transcripts by Reverse Transcription-PCR and Sequencing in a Case of AML With a FISH-Negative Cryptic MLL Rearrangement

Dear Editor The mixed lineage leukemia gene (MLL) on chromosome 11q23 is a frequent target of chromosomal translocations and rearrangements in childhood leukemia and adult therapy-related leukemia [1]. By using a commercially available dual-color FISH break-apart probe, almost all MLL rearrangements can be detected, including variant and cryptic forms [2-4]. We report a unique case of cryptic M...

Monosomal karyotype in Philadelphia chromosome-negative acute lymphoblastic leukemia

A monosomal karyotype (MK) is defined by the presence of two or more autosomal monosomies or one autosomal monosomy and one or more structural abnormality. MK has been shown to have a negative impact on survival in patients with acute myeloid leukemia, primary myelofibrosis and myelodysplastic syndromes. In several studies, the negative prognostic significance of MK in myeloid neoplasms persist...

Variant BCR-ABL1 fusion genes in adult Philadelphia chromosome-positive B-cell acute lymphoblastic leukemia